sec-deficient strains Search Results


sec-deficient strains  (Seca)
90
Seca sec-deficient strains
<t>TssL</t> insertion is dependent upon YidC and DnaK. Soluble proteins (soluble and peripherally associated membrane proteins; S) and integral inner membrane proteins (IM) were collected from various genetic backgrounds (WT-1, MC4100; secA , MM52 grown in restrictive conditions; <t>secB</t> , A443; tat , DADE; ftsY , IY28 grown in restrictive conditions; yidC , FTL10 grown in restrictive conditions; yidC + , FTL10 grown in permissive conditions; WT-2, BW25113; dnaKJ , PK101; dnaJ , GP108; dnaJ djlA , GP110; tig , A1091) and subjected to 12.5% acrylamide SDS-PAGE. The localization of the TolR and Flag TssL proteins was assessed by immunodetection using anti-TolR and anti-FLAG antibodies, respectively. Arrows indicate soluble fractions of strains in which the TssL protein is not properly inserted in the inner membrane. Molecular weight markers are indicated on the left.
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TssL insertion is dependent upon YidC and DnaK. Soluble proteins (soluble and peripherally associated membrane proteins; S) and integral inner membrane proteins (IM) were collected from various genetic backgrounds (WT-1, MC4100; secA , MM52 grown in restrictive conditions; secB , A443; tat , DADE; ftsY , IY28 grown in restrictive conditions; yidC , FTL10 grown in restrictive conditions; yidC + , FTL10 grown in permissive conditions; WT-2, BW25113; dnaKJ , PK101; dnaJ , GP108; dnaJ djlA , GP110; tig , A1091) and subjected to 12.5% acrylamide SDS-PAGE. The localization of the TolR and Flag TssL proteins was assessed by immunodetection using anti-TolR and anti-FLAG antibodies, respectively. Arrows indicate soluble fractions of strains in which the TssL protein is not properly inserted in the inner membrane. Molecular weight markers are indicated on the left.

Journal: MicrobiologyOpen

Article Title: The C-tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci-1 Type VI secretion system, is inserted by YidC

doi: 10.1002/mbo3.9

Figure Lengend Snippet: TssL insertion is dependent upon YidC and DnaK. Soluble proteins (soluble and peripherally associated membrane proteins; S) and integral inner membrane proteins (IM) were collected from various genetic backgrounds (WT-1, MC4100; secA , MM52 grown in restrictive conditions; secB , A443; tat , DADE; ftsY , IY28 grown in restrictive conditions; yidC , FTL10 grown in restrictive conditions; yidC + , FTL10 grown in permissive conditions; WT-2, BW25113; dnaKJ , PK101; dnaJ , GP108; dnaJ djlA , GP110; tig , A1091) and subjected to 12.5% acrylamide SDS-PAGE. The localization of the TolR and Flag TssL proteins was assessed by immunodetection using anti-TolR and anti-FLAG antibodies, respectively. Arrows indicate soluble fractions of strains in which the TssL protein is not properly inserted in the inner membrane. Molecular weight markers are indicated on the left.

Article Snippet: We did not observe TssL mis-localization in Sec-deficient ( secA , secB ) or SRP-deficient ( ftsY ) strains.

Techniques: Membrane, SDS Page, Immunodetection, Molecular Weight